Publications

2022

Characterization of the SARS-CoV-2 ExoN )nsp14ExoN-nsp10) complex: implications for its role in viral genome stability and inhibitor identification.

Baddock HT, Brolih S, Yosaatmadja Y, Ratnaweera M, Bielinski M, Swift LP, Cruz-Migoni A, Fan H, Keown JR, Walker AP, Morris GM, Grimes JM, Fodor E, Schofield CJ, Gileadi O, McHugh PJ.

Nucleic Acids Res 2022 Jan 17;gkab1303..

The C-terminal domains of the PB2 subunit of the influenza A virus RNA polymerase directly interact with cellular GTPase Rab11a.

Veler H, Fan H, Keown J, Sharps, Fournier M, Grimes JM, Fodor E.

J Virol 2022 Jan 12:jvi0197921.

Mapping inhibitory sites on the RNA polymerase of the 1918 pandemic influenza virus using nanobodies.

Keown JR, Zhu Z, Carrique L, Fan H, Walker AP, Serna Martin I, Pardon E, Steyaert J, Fodor E, Grimes JM.

Nat Commun 2022 Jan 11;13(1):251.

2021

The SARS-CoV-2 RNA polymerase is a viral RNA capping enzyme.

Walker AP, Fan H, Keown JR, Knight ML, Grimes JM, Fodor E.

Nucleic Acids Res 2021 Dec 16;49(22):13019-13030.

Structure of an H3N2 influenza virus nucleoprotein.

Knight ML, Fan H, Bauer DLV, Grimes JM, Fodor E, Keown JR.

Acta Crystallogr F Struct Biol Commun 2021 Jul 1;77(Pt 7):208-214.

A synergistic effect between 3' terminal noncoding and adjacent coding regions of influenza A virus HA segment on template preference.

Xiao Y, Zhang W, Pan M, Bauer DLV, Bi Y, Cao M, Fodor E, Deng T.

J Virol 2021 Jun 30:JVI0087821.

Identification of guanylyltransferase activity in the SARS-CoV-2 RNA polymerase.

Walker AP, Fan H, Keown JR, Grimes JM, Fodor E.

bioRxiv 2021 Mar 17;2021.03.17.435913.

Structural insights into RNA polymerases of negative-sense RNA viruses.

te Velthuis AJW, Grimes JM, Fodor E.

Nature Rev Microbiol 2021 May;19(5):303-318.

2020

Host ANP32A mediates the assembly of the influenza virus replicase.

Carrique L, Fan H, Walker AP, Keown JR, Sharps J, Staller E, Barclay WS, Fodor E, Grimes JM.

Nature 2020 Nov 18;587(7835):638-643.

Enisamium is a small molecule inhibitor of the influenza A virus and SARS-CoV-2 RNA polymerases.

Walker AP, Fan H, Keown JR, Margitich V, Grimes JM, Fodor E, te Velthuis AJW.

bioRxiv 2020 Jan 6;2020.04.21.053017.

Mutation of an influenza virus polymerase 3' RNA promoter binding site inhibits transcription elongation.

Walker AP, Sharps J, Fodor E.

J Virol. 2020 Jun 16;94(13):e00498-20.

Insight into the multifunctional RNA synthesis machine of rabies virus.

Fodor E.

Proc Natl Acad Sci U S A. 2020 Feb 25;117(8):3895-3897.

Structure and function of the influenza virus transcription and replication machinery.

Fodor E, te Velthuis AJW.

Cold Spring Harb Perspect Med. 2020 Sep 1;10(9):a038398.

2019

Design, synthesis, and biological evaluation of novel indols targeting the influenza PB2 cap binding region.

McGowan DC, Balemans W, Embrechts W, Motte M, Keown J, Buyck C, Corbera J, Funes M, Moreno L, Cooymans L,Tahri A, Eymard J, Stoops B, Strijbos R, Van den Berg J, Fodor E, Grimes J, Koul A, Jonckers THM, Raboisson PJMB, Guillemont J.

J Med Chem. 2019 Oct 14;62(21):9680-9690.

Structures of influenza A virus polymerase offer insight into viral genome replication.

Fan H, Walker AP, Carrique L, Keown JR, Serna Martin I, Karia D, Sharps J, Hengrung N, Pardon E, Steyaert J, Grimes JM, Fodor E.

Nature 2019 Sep 4;573(7773):287-290.

The structure of the influenza A virus genome.

Dadonaite B, Gilbertson B, Knight ML, Trifkovic S, Rockman S, Laederach A, Brown LE, Fodor E, Bauer DLV.

Nat Microbiol. 2019 Jul 22;4(11):1781-1789.

Biological properties of influenza A virus mutants with amino acid substitutions in the HA2 glycoprotein of the HA1/HA2 interaction region.

Jakubcová L, Vozárová M, Hollý J, Tomčíková K, Fogelová M, Polčicová K, Kostolanský F, Fodor E, Varečková V.

J Gen Virol. 2019 Sep;100(9):1282-1292.

Real-time analysis of single influenza virus replication complexes reveals large promoter-dependent differences in initiation dynamics.

Robb NC, te Velthuis AJW, Fodor E, Kapanidis AN.

Nucleic Acids Res. 2019 Jul 9;47(12):6466-6477.

Interplay between influenza virus and the host RNA polymerase II transcriptional machinery.

Walker AP, Fodor E.

Trends Microbiol. 2019 May;27(5):398-407.

2018

Mini viral RNAs act as innate immune agonists during influenza virus infection.

Te Velthuis AJW, Long JC, Bauer DLV, Fan RLY, Yen HL, Sharps J, Siegers JY, Killip MJ, French H, Oliva-Martín MJ, Randall RE, de Wit E, van Riel D, Poon LLM, Fodor E.

Nat Microbiol. 2018 Sep 17;3(11):1234-1242.

see also News & Views Riedl W, Gack, MU. Dusting for flu's fingerprints. Nat Microbiol. 2018 Nov; 3(11):1196-1197.

A mechanism for the activation of the influenza virus transcriptase.

Serna Martin I, Hengrung N, Renner M, Sharps J, Martínez-Alonso M, Masiulis M, Grimes JM, Fodor E.

Mol Cell. 2018 Jun 21;70(6):1101-1110.e4.

The surface-exposed PA(51-72)-loop of the influenza A virus polymerase is required for viral genome replication.

Nilsson-Payant BE, Sharps J, Hengrung N, Fodor E.

J Virol. 2018 Jun 6. pii: JVI.00687-18.

Influenza virus mounts a two-pronged attack on host RNA polymerase II transcription.

Bauer DLV, Tellier M, Martínez-Alonso M, Nojima T, Proudfoot NJ, Murphy S, Fodor E.

Cell Rep. 2018 May 15;23(7):2119-2129.e3.

Amino acid substitutions affecting aspartic acid 605 and valine 606 decrease the interaction strength between the influenza virus RNA polymerase PB2 '627' domain and the viral nucleoprotein.

Hsia HP, Yang YH, Szeto WC, Nilsson BE, Lo CY, Ng AK, Fodor E, Shaw PC.

PLoS One. 2018 Jan 16;13(1):e0191226.

2017

The role of the PB2 627-domain in influenza A virus polymerase function.

Nilsson BE, te Velthuis AT, Fodor E.

J Virol. 2017 Mar 13;91(7). pii: e02467-16.

Single-cell studies of IFN-β promoter activation by wildtype and NS1-defective influenza A viruses.

Killip MJ, Jackson D, Perez-Cidoncha M, Fodor E, Randall RE.

J Gen Virol. 2017 Mar;98(3):357-363.

2016

Moving on out: transport and packaging of influenza viral RNA into virions.

Lakdawala SS, Fodor E, Subbarao K.

Annu Rev Virol. 2016 Sep 29;3(1):411-417.

Single-molecule FRET reveals the pre-initiation and initiation conformations of the influenza virus promoter RNA.

Robb NC, te Velthuis AJ, Wieneke R, Tampé, Cordes T, Fodor E, Kapanidis AN.

Nucleic Acids Res. 2016 Dec 1;44(21):10304-10315.

The PB2 subunit of the influenza A virus polymerase is imported into the mitochondrial matrix.

Long JC, Fodor E.

J Virol. 2016 Sep 12;90(19):8729-38. Print 2016 Oct 1.

Influenza virus RNA polymerase: insights into the mechansims of viral RNA synthesis.

te Velthuis AJW, Fodor E.

Nat Rev Microbiol. 2016 Aug;14(8):479-93. Epub 2016 Jul 11.

Filamentous influenza viruses.

Dadonaite B, Vijyakrishnan S, Fodor E, Bhella D, Hutchinson EC.

J Gen Virol. 2016 Aug;97(8):1755-64. Epub 2016 Jun 30.

RNA-free and ribonucleoprotein-associated influenza virus polymerases directly bind the serine-5-phosphorylated carboxyl-terminal domain of host RNA polymerase II.

Martinez-Alonso M, Hengrung N, Fodor E.

J Virol. 2016 Jun 10;90(13):6014-21. Print 2016 Jul 1.

The role of the priming loop in influenza A virus RNA synthesis.

te Velthuis AJW, Robb NC, Kapanidis AN, Fodor E.

Nat Microbiol. 2016 May;1(5). pii: 16029. Epub 2016 Mar 21.

2015

Crystal structure of the RNA-dependent RNA polymerase from influenza C virus.

Hengrung N, El Omari K, Serna Martin I, Vreede FT, Cusack S, Rambo RP, Vonrhein C, Bricogne G, Stuart DI, Grimes JM, Fodor E.

Nature 2015 Nov 5;527(7576):114-7. Epub 2015 Oct 26.

Targeting importin-α7 as a therapeutic approach against pandemic influenza viruses.

Resa-Infante P, Paterson D, Bonet J, Otte A, Oliva B, Fodor E, Gabriel G.

J Virol. 2015 Sep;89(17):9010-20. Epub 2015 Jun 17.

Influenza virus activation of the interferon system.

Killip MJ, Fodor E, Randall RE.

Virus Res. 2015 Nov 2;209:11-22. Epub 2015 Feb 9.

Regulation of influenza A virus nucleoprotein oligomerization by phosphorylation.

Turrell L, Hutchinson EC, Vreede FT, Fodor E.

J Virol. 2015 Jan 15:89(2):1452-5. Epub 2014 Oct 29.

2014

Conserved and host-specific features of influenza virion architecture.

Hutchinson EC, Charles PD, Hester SS, Thomas B, Trudgian D, Martínez-Alonso M, Fodor E.

Nat Commun. 2014 Sep 16;5:4816.

Interactome analysis of the influenza A virus transcription/replication machinery identifies protein phosphatase 6 as a cellular factor required for efficient virus replication.

York A, Hutchinson EC, Fodor E.

J Virol. 2014 Nov;88(22):13284-99.

Single-molecule FRET reveals a corkscrew RNA structure for the polymerase-bound influenza virus promoter.

Tomescu AI, Robb NC, Hengrung N, Fodor E, Kapanidis AN.

Proc Natl Acad Sci U S A. 2014 Aug 12;111(32):E3335-42.

Molecular determinants of pathogenicity in the polymerase complex.

Gabriel G, Fodor E.

Curr Top Microbiol Immunol. 2014;385:35-60.

Influenza a virus assembly intermediates fuse in the cytoplasm.

Lakdawala SS, Wu Y, Wawrzusin P, Kabat J, Broadbent AJ, Lamirande EW, Fodor E, Altan-Bonnet N, Shroff H, Subbarao K.

PLoS Pathog. 2014 Mar 6;10(3):e1003971.

Host restriction of influenza virus polymerase activity by PB2 627E is diminished on short viral templates in a nucleoprotein-independent manner.

Paterson D, te Velthuis AJ, Vreede FT, Fodor E.

J Virol. 2014 Jan;88(1):339-44.

2013

Isolation and characterization of the positive-sense replicative intermediate of a negative-strand RNA virus.

York A, Hengrung N, Vreede FT, Huiskonen JT, Fodor E.

Proc Natl Acad Sci U S A. 2013 Nov 5;110(45):E4238-45.

Transport of the influenza virus genome from nucleus to nucleus.

Hutchinson EC, Fodor E.

Viruses. 2013 Oct 2;5(10):2424-46.

Uncoupling of influenza A virus transcription and replication through mutation of the unpaired adenosine in the viral RNA promoter.

te Velthuis AJ, Turrell L, Vreede FT, Fodor E.

J Virol. 2013 Sep;87(18):10381-4.

Biogenesis, assembly, and export of viral messenger ribonucleoproteins in the influenza A virus infected cell.

York A, Fodor E.

RNA Biol. 2013 Aug;10(8):1274-82.

The RNA polymerase of influenza A virus: mechanisms of viral transcription and replication.

Fodor E.

Acta Virol. 2013;57(2):113-22.

The role and assembly mechanism of nucleoprotein in influenza A virus ribonucleoprotein complexes.

Turrell L, Lyall JW, Tiley LS, Fodor E, Vreede FT.

Nat Commun. 2013;4:1591.

The virus genome and its replication.

Krug RM, Fodor E.

In: Textbook of Influenza. Webster RG, Monto AS, Braciale TJ, Lamb RA (eds.), 2nd Edition, Wiley-Blackwell, pp. 57-66.

2012

Emerging roles for the influenza A virus nuclear export protein (NEP).

Paterson D, Fodor E.

PLoS Pathog. 2012;8(12):e1003019.

Mapping the phosphoproteome of influenza A and B viruses by mass spectrometry.

Hutchinson EC, Denham EM, Thomas B, Trudgian DC, Hester SS, Ridlova G, York A, Turrell L, Fodor E.

PLoS Pathog. 2012;8(11):e1002993.

Identification of a novel splice variant form of the influenza A virus M2 ion channel with an antigenically distinct ectodomain.

Wise HM, Hutchinson EC, Jagger BW, Stuart AD, Kang ZH, Robb N, Schwartzman LM, Kash JC, Fodor E, Firth AE, Gog JR, Taubenberger JK, Digard P.

PLoS Pathog. 2012;8(11):e1002998.

Pseudotyped influenza A virus as a vaccine for the induction of heterotypic immunity.

Powell TJ, Silk JD, Sharps J, Fodor E, Townsend AR.

J Virol. 2012 Dec;86(24):13397-406.

Nuclear import of the influenza A virus transcriptional machinery.

Hutchinson EC, Fodor E.

Vaccine. 2012 Dec 7;30(51):7353-8.

Influenza polymerase activity correlates with the strength of interaction between nucleoprotein and PB2 through the host-specific residue K/E627.

Ng AK, Chan WH, Choi ST, Lam MK, Lau KF, Chan PK, Au SW, Fodor E, Shaw PC.

PLoS One. 2012;7(5):e36415.

The accumulation of influenza A virus segment 7 spliced mRNAs is regulated by the NS1 protein.

Robb NC, Fodor E.

J Gen Virol. 2012 Jan;93(Pt 1):113-8.

2011

Stabilization of influenza virus replication intermediates is dependent on the RNA-binding but not the homo-oligomerization activity of the viral nucleoprotein.

Vreede FT, Ng AK, Shaw PC, Fodor E.

J Virol. 2011 Nov;85(22):12073-8.

Characterization of the interaction between the influenza A virus polymerase subunit PB1 and the host nuclear import factor Ran-binding protein 5.

Hutchinson EC, Orr OE, Man Liu S, Engelhardt OG, Fodor E.

J Gen Virol. 2011 Aug;92(Pt 8):1859-69.

The influenza A virus NS1 protein interacts with the nucleoprotein of viral ribonucleoprotein complexes.

Robb NC, Chase G, Bier K, Vreede FT, Shaw PC, Naffakh N, Schwemmle M, Fodor E.

J Virol. 2011 May;85(10):5228-31.

Cellular cap-binding proteins associate with influenza virus mRNAs.

Bier K, York A, Fodor E.

J Gen Virol. 2011 Jul;92(Pt 7):1627-34.

Differential use of importin-α isoforms governs cell tropism and host adaptation of influenza virus.

Gabriel G, Klingel K, Otte A, Thiele S, Hudjetz B, Arman-Kalcek G, Sauter M, Shmidt T, Rother F, Baumgarte S, Keiner B, Hartmann E, Bader M, Brownlee GG, Fodor E, Klenk HD.

Nat Commun. 2011 Jan 18;2:156.